Human Group IVC Phospholipase A2(cPLA2γ)

Arachidonate release and prostaglandin production by group IVC phospholipase A2 (cytosolic phospholipase A2gamma).

While the role of the group IVA Ca(2+)-dependent cytosolic phospholipase A(2)alpha (cPLA(2)alpha) in arachidonic acid (AA) metabolism has been well documented, that of its paralogue, Ca(2+)-independent group IVC PLA(2) (cPLA(2)gamma), has remained uncertain. Here we show, using a transfection strategy, that cPLA(2)gamma has the ability to increase the spontaneous and stimulus-induced release of...

Group IVC cytosolic phospholipase A 2 is farnesylated and palmitoylated in mammalian cells

Cytosolic phospholipase A 2 (cPLA 2 ) is a member of the group IV family of intracellular phospholipase A 2 enzymes, but unlike the well-studied cPLA 2 , it is constitutively bound to membrane and is calcium independent. cPLA 2 contains a C-terminal CaaX sequence and is radiolabeled by mevalonic acid when expressed in cPLA 2 -deficient immortalized lung fibroblasts (IMLF / ). The radiolabel ass...

Induction of group IVC phospholipase A2 in allergic asthma: transcriptional regulation by TNFα in bronchoepithelial cells.

Airway inflammation in allergen-induced asthma is associated with eicosanoid release. These bioactive lipids exhibit anti- and pro-inflammatory activities with relevance to pulmonary pathophysiology. We hypothesized that sensitization/challenge using an extract from the ubiquitous fungus Aspergillus fumigatus in a mouse model of allergic asthma would result in altered phospholipase gene express...

Leptin augments alveolar macrophage leukotriene synthesis by increasing phospholipase activity and enhancing group IVC iPLA2 (cPLA2gamma) protein expression.

Leptin is a hormone secreted by adipocytes in correlation with total body fat mass. In addition to regulating energy homeostasis, leptin modulates immune functions such as macrophage phagocytosis and cytokine synthesis. Previously, we reported defective leukotriene synthesis in macrophages from leptin-deficient mice that could be restored with exogenous leptin. In the present study, we utilized...

Human eosinophil group IID secretory phospholipase A2 causes surfactant dysfunction.